Abstract:Objective To investigate the safety of medical self-crosslinked sodium hyaluronic gel (HyaRegen/SPI) on the prevention of epidural adhesions after laminectomy by using cortical somatosensory evoked potentials (CSEP) and motor scoring.
Methods Twenty-four New Zealand white rabbits with lumbar laminectomy at L5 were randomly divided into four groups: group A as control group, in which only saline was used to flush the laminectomy area before wound closure, group B, C and D as treated groups, in which the exposed dura were covered with 0.5, 0.75 and 1.0 ml of the medical self-crosslinked sodium hyaluronic gel (HyaRegen/SPI) respectively before wound closure. The CSEP with latency was monitored and recorded after anesthesia, laminectomy and wound closure and at 8 weeks post-surgery. Motor scoring was also conducted at the time of pre-surgery and also 1 day and 8 weeks post-surgery.
Results There were no significant difference in CSEP latency among groups after anesthesia and laminectomy (P > 0.05). The latency in group C and D (covered with 0.75 and 1.0 ml HyaRegen/SPI respectively) was significantly prolonged (P < 0.05) after wound closure, while there was no prolongation in group A (control group) and B (covered with 0.5 ml HyaRegen/SPI) (P > 0.05). However, the latency in all groups was all in the normal range (P > 0.05) at 8 weeks post-surgery. The evaluation of motor scores revealed that all animals had a normal motor function before surgery and at 1 day and 8 weeks post-surgery, except a lower motor score in group C and D on 1th day post-surgery.
Conclusion The CSEP is a sensitive indicator of spinal cord injury. There is a dosage limitation in the application of agent on the prevention of epidural adhesions after laminectomy. The application of medical self-crosslinked sodium hyaluronic gel (HyaRegen/SPI) 0.5 ml do not affect the electrophysiological function of spine and motor function in this animal model.
收稿日期: 2011-12-27
通讯作者:
郭全义
E-mail: doctorguo@163.com
引用本文:
梁文锴, 郭全义, 韩树峰, 张莉, 彭江, 刘舒云, 梁增义, 许文静, 卢世璧. 医用自交联透明质酸钠凝胶预防兔硬膜外粘连的安全性研究[J]. 《中国医药生物技术》杂志, 2012, 7(1): 30-36.
LIANG Wen-Kai, GUO Quan-Yi, HAN Shu-Feng, ZHANG Li, PENG Jiang, LIU Shu-Yun, LIANG Zeng-Yi, XU Wen-Jing, LU Shi-Bi. Safety study on medical self-crosslinked sodium hyaluronic gel for prevention of epidural adhesions in rabbits. , 2012, 7(1): 30-36.