Abstract:Objective To investigate whetherlentinan (LNT) could induce the production of IL-12 mediated by Dectin-1 in human THP-1 monocytes (a human acute monocytic leukemia cell line).
Methods Human THP-1 cells were challenged with LNT in vitro. The mRNA expressions of Dectin-1, IL-12p35 and IL-12p40 in THP-1 cells treated with LNT were assayed by real time reverse transcription polymerase chain reaction. The secretion of IL-12 were measured by enzyme-linked immunosorbent assay. The effect of the Dectin-1 inhibitor laminarin on the production of IL-12 was determined in THP-1 cells induced by LNT.
Results LNT increased the mRNA expressions of Dectin-1, IL-12p35, IL-12p40 and secretion of IL-12 in THP-1 cells. Pretreatment with Dectin-1 inhibitor laminarin significantly inhibited the LNT-induced production of IL-12 in THP-1 cells.
Conclusion Dectin-1 may play an important role in LNT-induced production of IL-12 in human THP-1 monocytes.
陈青, 陈晓, 孙俊, 曾容, 胡素泉, 李岷, 刘维达. Dectin-1介导香菇多糖诱导人单核细胞系THP-1细胞产生IL-12的研究[J]. 《中国医药生物技术》杂志, 2012, 7(1): 26-29.
CHEN Qing, CHEN Xiao, SUN Jun, ZENG Rong, HU Su-Quan, LI Min, LIU Wei-Da. Lentinan induces the production of IL-12 by mediated by Dectin-1 in human THP-1 monocytes. , 2012, 7(1): 26-29.