1.Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029,China; 2. Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences; 3.Department of Vascular Surgery, China-Japan Friendship Hospital Cancer Institute; 4. Chinese Academy of Medical Sciences Institute of Clinical Medical Sciences
Objeetive To establish an animal model of human cavernous angioma for drug screening. Methods Endothelial cells derived from human cavernous angioma (HCAEC) were isolated and purified by Ulex europaeus agglutinin-1(UEA-1)-conjugated magnetic beads. An animal model of human cavernous angioma was established by co-inoculating HCAEC and human liver cancer cells (Bel-7402) into immune-deficient nude mice subcutaneously. GFP-tagging and histopathological examinations were used to analyze the source of endothelial cells and pathological features in the angioma tissue. In addition, drugs for angioma were screened by HCAEC assay in vitro, and using the angioma model in vivo. Results An angioma-like structure was found on the site of co-inoculation of HCAEC (2.5×106) and Bel-7402 cells (5×105) 7－9 days after the transplantation. The histological feature of the angioma-like tissues was similar to that of a human angioma. GFP-tagging showed green fluorescence on the vascular wall of the angioma-like tissues, suggesting that the endothelial cells came from the inoculated HCAEC. Drug screening found that 876-3, an extract from a Chinese traditional medicine, was an effective drug for inhibiting the proliferation of HCAEC in vitro, and for treating the angioma in vivo.
Conclusions An animal model of human angioma can be established by co-inoculating HCAEC and Bel-7402 cells into immune-deficient nude mice, and this animal model can be used in drug screening for angioma.
ZHANG Wen-jian;WU Lian-qiu;GU Feng;YE Li-ya;XIN Yu-ling;YANG Zhi-hua;LOU Jin-nin.Establishment of an animal model of human cavernous angioma for drug screening[J] , 2007,V2(1): 13-13-17
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