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| Preparation of collagen scaffold for rotary cell culture system and effect on repairing cartilage damages of it with adhension of chondrocytes |
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Abstract Objective Repair of focal articular cartilage injury may be solved by tissue engineering. The preparation of proper scaffold is one of the most important problems to be solved. The aim of the study was to construct a kind of collagen scaffold, co-culture with L929 cells in rotary cell culture system, and implant into animal models with cartilage damage.
Methods Collagen scaffold was prepared from achilles tendon of pigs. Collagen scaffold was cut into small pieces with the size of 1 mm3 and co-cultured with L929 cells in rotary cell culture system, and the cytotoxicity and biocompatibility were tested. Rabbit articular cartilage damage models were prepared and divided into 2 groups: the experimental group treated by scaffold with chondrocytes (n = 8) and control group (n = 6) without treatment. After 4 or 12 weeks, the specimens were extracted after surgery. The effects on repairing cartilage injury were examined with haematoxylin and erosin staining.
Results The collagen scaffold exhibited good biocompatibilities, and had no cell toxicity. It was proper to co-culture with chondrocytes in rotary cell culture system. The collagen scaffold with adhension of chondrocytes showed effect of repairing cartilage damage, but still could not completely reconstruct the damage.
Conclusion The collagen scaffold with adhension of chondrocytes can repair cartilage damage in a short period, but it shows less potent effect in a long period, which may be correlated with the rapid degradation of the scaffold. The method of collagen scaffold preparation should be improved in our future work.
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Received: 06 June 2012
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2012, Vol. 7 
