Separation, purification and structural identification of an active nematicidal component from the fermentation broth of Endophytic streptomyces I07A-01824 from Mangrove ecosystem
Abstract:Objective To establish an efficient in vitro model for screening of new nematicidal compounds, and to separate, purify and identify the structure of an active nematicidal component from the fermentation broth of Endophytic actinomycets I07A-01824.
Methods Silica gel column chromatography and HPLC were used to separate and purify the active component. Its structure was identified by spectroscopic analyses.
Results The active component was purified and its structure was identified as an unsaturated fatty acid-tetradeca-5,8-dienoic acid. It displays the cytotoxic activity to Caenorhabditis elegans at 27.3 mg/L. Its LC50 is 162.8 mg/L in 24 h.
Conclusions The unsaturated fatty acid was first isolated from the strain of Endophytic actinomycets with nematicidal activity.
陶玲, 旭格拉?哈布丁, 韩宁宁, 余生艳, 闫蕾蕾, 栾迎春, 刘少伟, 郭琳, 蒋忠科, 余利岩, 孙承航. 红树林植物内生放线菌I07A-01824发酵液中杀线虫活性成分的分离、纯化与鉴定[J]. 《中国医药生物技术》杂志, 2012, 7(1): 5-8.
TAO Ling, HABDEN Xugela, HAN Ning-Ning, YU Sheng-Yan, YAN Lei-Lei, LUAN Ying-Chun, LIU Shao-Wei, GUO Lin, JIANG Zhong-Ke, YU Li-Yan, SUN Cheng-Hang. Separation, purification and structural identification of an active nematicidal component from the fermentation broth of Endophytic streptomyces I07A-01824 from Mangrove ecosystem. , 2012, 7(1): 5-8.