Abstract：Objective To determine the role of sphingosine kinase (SPK) in hepatocyte carcinoma (HCC) metastasis. Methods The expression and activation of SPK in Ad-SPK infected LM-3 HCC cell line was detected by Western blot; and HCC cells migration was evaluated by Transwell and scar-healing experiments. The impact of SPK on HUVEC tube morphologenesis was examined by in vitro tube formation assay. The SPK-overexpressing nude mice model was established to access HCC metastasis in vivo. Results SPK did not influence significantly HCC cells migration in vitro; HUVEC tube formation was stimulate by Ad-SPK. Ad-SPK accelerated the tumor growth and metastasis in vivo. Conclusion Sphingosine kinase promotes the HCC metastasis via regulating angiogenesis.
赛岩, 代学强, 白纪民, 刘勇, 常菁, 范礼斌, 崔春萍. 鞘氨醇激酶通过调控血管发生促进肝癌转移[J]. 《中国医药生物技术》杂志, 2012, 7(3): 171-177.
SAI Yan, DAI Xue-Qiang, BAI Ji-Min, LIU Yong, CHANG Jing, FAN Li-Bin, CUI Chun-Ping. Sphingosine kinase promote HCC metastasis via regulation of angiogenesis. , 2012, 7(3): 171-177.
2012, Vol. 7 
