Abstract：
Objective To observe the effects of SNG-162 on cell proliferation and expression of estrogen receptor ER-α36 in human endometrial cancer cell line HEC-1B.
Methods HEC-1B cells were cultured in vitro. HEC-1B cell proliferation was measured by using MTT method and the gene expression of estrogen receptor ER-α66 and ER-α36 were analyzed by the reverse transcriptase polymerase chain reaction (RT-PCR).
Results HEC-1B cell proliferation was significantly inhibited by SNG-162. The inhibitory rates were gradually increased with the concentration and time. After 72 hours, the IC50 value of SNG-162 was 5.26 μmol/L. To compare with the control group, SNG-162 alone or SNG-162 combined with cisplatin groups can significantly reduce the ER-α36 gene expression (P < 0.05, P < 0.01). SNG-162 combined with cisplatin also significantly reduced ER-α66 expression (P < 0.01).
Conclusion SNG-162 inhibits the proliferation of endometrial cancer cells, which may be related to the inhibition of estrogen receptor ER-α36 gene expression.
彭瑶, 张文谨, 连增林, 靳冉, 李晋生, 孟坤. 阿可拉定对子宫内膜癌HEC-1B细胞雌激素受体表达的影响[J]. 《中国医药生物技术》杂志, 2012, 7(2): 106-109.
PENG Yao, ZHANG Wen-Jin, LIAN Zeng-Lin, JIN Ran, LI Jin-Sheng, MENG Kun. The effects of Icaritin on the expression of estrogen receptor ER-α36 in endometrial cancer cell line. , 2012, 7(2): 106-109.
2012, Vol. 7 
