Abstract:
Objective To generate the high level expression transgenic HPPCn mice, an animal model for the study of its function and effects on Hepatocellular carcinoma.
Methods Transgenicmice were generated by pro-nuclear microinjection and embryo transplantation. Positive founder mice were identified by using both PCR and Southern blot. The expression of HPPCn in the liver tissues from wild type mice or transgenic mice was analyzed by using Western blot and realtime-PCR. The pathologic changes of the liver were analyzed by TEM and microscopy.
Results Two founder transgenic mice were obtained by microinjection, exogenous HPPCn gene existed in the liver tissues, and stable passage of the gene in the transgenic mice was achieved. The expression of HPPCn was up-regulated in the liver tissue of transgenic mice and there was no adverse effect on mice.
Conclusions HPPCn is involved in the occurrence and development of hepatocellular carcinoma. When its expression is raised, it can be used to investigate the effect of HPPCn gene in vivo levels.